Cyclophilin B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24770

Novus Biologicals, part of Bio-Techne
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NBP2-24770
NBP2-24770SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Porcine, Bovine, Canine, Equine, Primate

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Cyclophilin B Antibodies »

Product Specifications

Immunogen

The portions of amino acids range between 100-228 of human Cyclophilin B was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 84% in Chicken.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclophilin B Antibody

Knockout Validated: Cyclophilin B Antibody [NBP2-24770]

Knockout Validated: Cyclophilin B Antibody [NBP2-24770]

Knockout Validated: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Cyclophilin B Antibody [NBP2-24770]

Western Blot: Cyclophilin B Antibody [NBP2-24770]

Western Blot: Cyclophilin B Antibody [NBP2-24770] - analysis of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using this antibody. 5 and 2 ug/ml, respectively. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for Cyclophilin B Antibody

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27-34), a PPIase domain (aa 47-204) and a C-terminal ER retention motif (aa 213-216). Over aa 34-216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

UniProt

P23284

Additional Cyclophilin B Products

Product Documents for Cyclophilin B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclophilin B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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