CYFIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16060

Novus Biologicals, part of Bio-Techne
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NBP2-16060
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYFIP1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CYFIP1. The exact sequence is proprietary.

Localization

Cytoplasm; perinuclear region; Cell projection; lamellipodium; ruffle; Cell junction; synapse; synaptosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYFIP1 Antibody

Western Blot: CYFIP1 Antibody [NBP2-16060]

Western Blot: CYFIP1 Antibody [NBP2-16060]

Western Blot: CYFIP1 Antibody [NBP2-16060] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CYFIP1 Antibody [NBP2-16060]

Immunocytochemistry/ Immunofluorescence: CYFIP1 Antibody [NBP2-16060]

Immunocytochemistry/Immunofluorescence: CYFIP1 Antibody [NBP2-16060] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYFIP1 stained by CYFIP1 antibody [N1N2], N-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060]

Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060]

Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
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Applications for CYFIP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CYFIP1

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.

Alternate Names

cytoplasmic FMR1 interacting protein 1, cytoplasmic FMR1-interacting protein 1, FLJ45151, KIAA0068cytoplasmic FMRP interacting protein 1, p140sra-1, selective hybridizing clone, SHYC, Specifically Rac1-associated protein 1, SRA1, sra-1

Gene Symbol

CYFIP1

UniProt

Q7L576 (Human)

Additional CYFIP1 Products

Product Documents for CYFIP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYFIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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