CYLD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56461

Novus Biologicals, part of Bio-Techne
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NB100-56461
NB100-56461SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Feline (100%), Mouse (100%), Primate (100%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CYLD Antibodies »

Product Specifications

Immunogen

A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYLD Antibody

Western Blot: CYLD Antibody [NB100-56461]

Western Blot: CYLD Antibody [NB100-56461]

Western Blot: CYLD Antibody [NB100-56461] - Analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461]

Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461]

Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461]

Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461]

Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using this antibody at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
View 1 more images

Applications for CYLD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-10 ug/ml

Simple Western

1:50

Western Blot

2-4 ug/ml
Application Notes
Human CYLD is a 956 amino acid protein and the approximate theoretical size is 105 kD.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYLD

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

Long Name

Cylindromatosis

Alternate Names

BRSS, CDMT, CYLD1, EAC, MFT1, SBS, TEM, USPL2

Entrez Gene IDs

1540 (Human); 74256 (Mouse); 312937 (Rat)

Gene Symbol

CYLD

UniProt

Q9NQC7 (Human)

Additional CYLD Products

Product Documents for CYLD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CYLD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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