Cynomolgus Monkey IL-23R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB106243

R&D Systems, part of Bio-Techne
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MAB106243-100
MAB106243-SP
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Key Product Details

Species Reactivity

Cynomolgus Monkey

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1030030

View all IL-23R Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived Cynomolgus monkey IL-23R
Gly24-Asp353
Accession # XP_005543141.1

Specificity

Detects Cynomolgus monkey IL-23R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Cynomolgus Monkey IL-23R Antibody

IL-23R in Human Lymph Node.

IL-23R was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Cynomolgus Monkey IL-23R Monoclonal Antibody (Catalog # MAB106243) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Cynomolgus Monkey IL-23R Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). Human IL-23R cDNA encodes a 629 amino acids (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12R beta 2. IL-23R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23R is located on human chromosome 1 within 150 kb of IL-12R beta 2. Based on quantitative real-time PCR, human IL-23R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23R, respectively.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and Frucht, D.M. (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  6. Schroder, J. et al. (2015) J. Biol. Chem. 290:359.

Long Name

Interleukin 23 Receptor

Alternate Names

IL-23 R, IL23R

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Gene Symbol

IL23R

UniProt

XP_005543141.1

Additional IL-23R Products

Product Documents for Cynomolgus Monkey IL-23R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cynomolgus Monkey IL-23R Antibody

For research use only

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