CYP11A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85368

Novus Biologicals, part of Bio-Techne
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NBP1-85368
NBP1-85368-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rhesus Macaque

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP11A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA

Reactivity Notes

Rhesus monkey reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP11A1 Antibody

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368]

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368]

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.
Western Blot: CYP11A1 Antibody [NBP1-85368]

Western Blot: CYP11A1 Antibody [NBP1-85368]

Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody [NBP1-85368]

Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody [NBP1-85368]

Immunocytochemistry/Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a verified customer review.
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Applications for CYP11A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. CYP11A1 antibody validated for ICC/IF, WB from verified customer reviews.

Reviewed Applications

Read 4 reviews rated 5 using NBP1-85368 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP11A1

Alternate Names

Cholesterol desmolase, cholesterol monooxygenase (side-chain-cleaving), cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), cytochrome P450, family 11, subfamily A, polypeptide 1, cytochrome P450C11A1, EC 1.14.15, EC 1.14.15.6, steroid 20-22-lyase, subfamily XIA (cholesterol side chain cleavage)

Gene Symbol

CYP11A1

Additional CYP11A1 Products

Product Documents for CYP11A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYP11A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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