Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N8-P6E4*H8

Format

BSA Free

Concentration

1 mg/ml

View all CYP46A1 Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide- PVLCTLRPR (C-terminal sequence)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The clone number is listed as N8-P6E4*H8 in the scientific literature.

Scientific Data Images for CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

Western Blot: CYP46A1 Antibody (N8-P6E4*H8)BSA Free [NBP2-50194]

Western Blot: CYP46A1 Antibody (N8-P6E4*H8)BSA Free [NBP2-50194]

Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.
Immunohistochemistry: CYP46A1 Antibody (N8-P6E4*H8) - BSA Free [NBP2-50194]

Immunohistochemistry: CYP46A1 Antibody (N8-P6E4*H8) - BSA Free [NBP2-50194]

Immunohistochemistry: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - Shows strong cytoplasmic staining in metastatic colorectal cancer (A) and primary colorectal (B) compared to normal colon mucosa (C).

Applications for CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s):WB - overexpression lysates

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP46A1

CYP46A1 (cytochrome P450, family 46, subfamily A, polypeptide 1) belongs to the cytochrome P450 family which consist of a variety of enzymes that are involved in catalyzing reactions. CYP46A1 is expressed in the brain and is involved in turnover of cholesterol through the conversion of cholesterol to 24S-hydroxycholesterol. CYP46A1 is known to have interactions with CH25H, CYP27A1, CYP39A1, CYP7A1 and CEL. Recent studies of CYP46A1 have been conducted in relation to Alzheimer's disease.

Alternate Names

CH24H, cholesterol 24-hydroxylase, CYP46CP46, Cytochrome P450 46A1, cytochrome P450, family 46, subfamily A, polypeptide 1, cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase), EC 1.14.13.98

Gene Symbol

CYP46A1

Additional CYP46A1 Products

Product Documents for CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CYP46A1 Antibody (N8-P6E4*H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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