Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # M17-P3F2

Format

BSA Free

Concentration

1 mg/ml

View all CYP7B1 Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptide IQYPDSDVL (C-terminal sequence)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Scientific Data Images for CYP7B1 Antibody (M17-P3F2) - BSA Free

Western Blot: CYP7B1 Antibody (M17-P3F2)BSA Free [NBP2-50217]

Western Blot: CYP7B1 Antibody (M17-P3F2)BSA Free [NBP2-50217]

Western Blot: CYP7B1 Antibody (M17-P3F2) [NBP2-50217] - Western blot was performed using anti-Cytochrome P450 7B1 [M17-P3F2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.
Immunohistochemistry-Paraffin: CYP7B1 Antibody (M17-P3F2) - BSA Free [NBP2-50217]

Immunohistochemistry-Paraffin: CYP7B1 Antibody (M17-P3F2) - BSA Free [NBP2-50217]

Immunohistochemistry-Paraffin: CYP7B1 Antibody (M17-P3F2) [NBP2-50217] - Shows positive cytoplasmic immunostaining in normal liver (A) which is expected as CYP7B1 is involved in bile acid synthesis. Primary colorectal (B) and metastatic colorectal tumors (C) showed stronger staining compared to normal colon mucosa (D).

Applications for CYP7B1 Antibody (M17-P3F2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): IHC: formalin-fixed, paraffin-embedded human liver sections WB: pooled human liver microsomes.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP7B1

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-alpha-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25- and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.

Alternate Names

CBAS3, CP7B, Cytochrome P450 7B1, cytochrome P450, family 7, subfamily B, polypeptide 1, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1,25-hydroxycholesterol 7-alpha-hydroxylase, EC 1.14.13.100, Oxysterol 7-alpha-hydroxylase, oxysterol 7alpha-hydroxylase, spastic paraplegia 5A (autosomal recessive), SPG5A

Gene Symbol

CYP7B1

Additional CYP7B1 Products

Product Documents for CYP7B1 Antibody (M17-P3F2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CYP7B1 Antibody (M17-P3F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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