Cystathionase Antibody (2E12-1C10)
Novus Biologicals, part of Bio-Techne | Catalog # H00001491-M02
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E12-1C10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 22419888). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
H00001491-M02
Specificity
CTH - cystathionase (cystathionine gamma-lyase)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Cystathionase Antibody (2E12-1C10)
![Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02]](https://resources.bio-techne.com/images/products/Cystathionase-Antibody-2E12-1C10-Western-Blot-H00001491-M02-img0004.jpg "Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02]")
Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02]
Western Blot: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - CTH monoclonal antibody (M02), clone 2E12-1C10 Analysis of CTH expression in K-562.
Sandwich ELISA: Cystathionase Antibody (2E12-1C10) [H00001491-M02] - Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.
Applications for Cystathionase Antibody (2E12-1C10)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cystathionase
Alternate Names
cystathionase (cystathionine gamma-lyase), cystathionine gamma-lyase, cysteine desulfhydrase, EC 4.4.1, EC 4.4.1.1, Gamma-cystathionase, homoserine deaminase, homoserine dehydratase, MGC9471
Entrez Gene IDs
1491 (Human)
Gene Symbol
CTH
OMIM
219500 (Human)
UniProt
Additional Cystathionase Products
Product Documents for Cystathionase Antibody (2E12-1C10)
Product Specific Notices for Cystathionase Antibody (2E12-1C10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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