Cystathionase Antibody (4E1-1B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001491-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Guinea Pig

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E1-1B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cystathionase Antibodies »

Product Specifications

Immunogen

CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS

Reactivity Notes

Rat reported in scientific literature (PMID: 21571312). Mouse reported in scientific literature (PMID: 24951966)

Localization

Cytoplasmic

Specificity

CTH - cystathionase (cystathionine gamma-lyase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cystathionase Antibody (4E1-1B7)

Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01] - Analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M01), clone 4E1-1B7.Lane 1: CTH transfected lysate (Predicted MW: 44.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Immunohistochemistry-Paraffin: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Immunohistochemistry-Paraffin: Cystathionase Antibody (4E1-1B7) [H00001491-M01] - Immunoperoxidase of monoclonal antibody to CTH ( Cat # H00001491-M01 ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml]
Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01]

Western Blot: Cystathionase Antibody (4E1-1B7) [H00001491-M01] - CTH expression in K-562 ( Cat # L009V1 ).
View 3 more images

Applications for Cystathionase Antibody (4E1-1B7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA. IHC and ICC/IF reported in scientific literature (PMID: 23285278).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cystathionase

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

cystathionase (cystathionine gamma-lyase), cystathionine gamma-lyase, cysteine desulfhydrase, EC 4.4.1, EC 4.4.1.1, Gamma-cystathionase, homoserine deaminase, homoserine dehydratase, MGC9471

Entrez Gene IDs

1491 (Human)

Gene Symbol

CTH

OMIM

219500 (Human)

UniProt

AAH15807 (Human)

Additional Cystathionase Products

Product Documents for Cystathionase Antibody (4E1-1B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cystathionase Antibody (4E1-1B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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