Cystatin-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47219

Novus Biologicals, part of Bio-Techne
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NBP1-47219
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cystatin-8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPKKNETGVLRK corresponding to internal region according to NP_005483.1.

Epitope

Internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cystatin-8 Antibody

Western Blot: Cystatin-8 Antibody [NBP1-47219]

Western Blot: Cystatin-8 Antibody [NBP1-47219]

Western Blot: Cystatin-8 Antibody [NBP1-47219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with (0.3ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for Cystatin-8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human CST8 (myc and DYKDDDDK tagged), a band of approx. 23 kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cystatin-8

Cystatin 8, also referred to as CST8, has a 142 amino acid isoform that is 16 kDa, and is involved in reproduction, especially pertaining to sperm development and maturation. Disease research is being conducted between Cystatin 8 and several diseases and disorders to determine the relation between the two. Some of these disorders include epididymitis, cerebral hemorrhage, melanoma, t-cell leukemia, cerebritis, and choriocarcinoma. This protein is not linked to any pathways, and is not known to interact with any other proteins at this time.

Alternate Names

CRESCystatin-related epididymal spermatogenic protein, cystatin 8 (cystatin-related epididymal specific), cystatin-8, cystatin-related epididymal-specific

Entrez Gene IDs

10047 (Human)

Gene Symbol

CST8

UniProt

NP_005483.1 (Human)

Additional Cystatin-8 Products

Product Documents for Cystatin-8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cystatin-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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