Cystatin-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49834

Novus Biologicals, part of Bio-Techne
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NBP1-49834
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cystatin-8 Antibodies »

Product Specifications

Immunogen

Peptide with sequence STNEICAIQENSK corresponding to internal region according to NP_005483.1.

Epitope

STNEICAIQENSK - internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cystatin-8 Antibody

Western Blot: Cystatin-8 Antibody [NBP1-49834]

Western Blot: Cystatin-8 Antibody [NBP1-49834]

Western Blot: Cystatin-8 Antibody [NBP1-49834] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for Cystatin-8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human CST8 (MYC and DYKDDDDK tagged), a band of approx. 23 kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cystatin-8

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing identified in mouse is suggested in human based on EST evidence but the full-length nature of putative variants has not been determined.

Alternate Names

CRESCystatin-related epididymal spermatogenic protein, cystatin 8 (cystatin-related epididymal specific), cystatin-8, cystatin-related epididymal-specific

Entrez Gene IDs

10047 (Human)

Gene Symbol

CST8

UniProt

NP_005483.1 (Human)

Additional Cystatin-8 Products

Product Documents for Cystatin-8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cystatin-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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