Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-CSTB-3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Cystatin B Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cystatin B/Stefin B protein (Uniprot: P04080)

Localization

Cytoplasm and nucleus

Marker

Marker of Epithelial Ovarian Cancer Progression

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07788).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free [NBP3-08538]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free [NBP3-08538]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free [NBP3-08538] - Formalin-fixed, paraffin-embedded human lung stained with Cystatin B/Stefin B Mouse Monoclonal Antibody (CPTC-CSTB-3).

Applications for Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cystatin B

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Alternate Names

CSTB, Stefin B

Gene Symbol

CSTB

Additional Cystatin B Products

Product Documents for Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cystatin B/Stefin B Antibody (CPTC-CSTB-3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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