Cystatin B/Stefin B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85430

Novus Biologicals, part of Bio-Techne
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NBP1-85430
NBP1-85430-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cystatin B Antibodies »

Product Summary for Cystatin B/Stefin B Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cystatin B/Stefin B Antibody

Western Blot: Cystatin B/Stefin B Antibody [NBP1-85430]

Western Blot: Cystatin B/Stefin B Antibody [NBP1-85430]

Western Blot: Cystatin B/Stefin B Antibody [NBP1-85430] - Analysis in human cell lines A-431 and U-251MG using anti-CSTB antibody. Corresponding CSTB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-85430]

Immunocytochemistry/ Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-85430]

Immunocytochemistry/Immunofluorescence: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430]

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.
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Applications for Cystatin B/Stefin B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cystatin B

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Alternate Names

CSTB, Stefin B

Gene Symbol

CSTB

Additional Cystatin B Products

Product Documents for Cystatin B/Stefin B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cystatin B/Stefin B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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