Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoassay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Cyst24cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cystatin C Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Cystatin C purified from human urine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cystatin C Antibody (Cyst24cc)

ELISA: Cystatin C Antibody (Cyst24cc) [NB100-62127]

ELISA: Cystatin C Antibody (Cyst24cc) [NB100-62127]

ELISA: Cystatin C Antibody (Cyst24) [NB100-62127] - Calibration curve One-step fluoroimmunoassay in streptavidin coated plates. Capture MAbs: Cyst24 and Cyst23 are biotinylated (200 ng/well). Detection MAbs: Cyst10, Cyst19, Cyst28 or Cyst13 are Eu3+- labelled (200 ng/ml). Incubation volume 100 ul.
Sandwich ELISA: Cystatin C Antibody (Cyst24) [NB100-62127] - Titration curve of pooled normal serum in Cyst24. Cyst10- Eu3plus sandwich fluoroimmunoassay.

Applications for Cystatin C Antibody (Cyst24cc)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection):

Cyst24 - Cyst19, (NB100-62127/NBP1-05766)
Cyst24 - Cyst28, (NB100-62127/NBP1-05765)
Cyst23 - Cyst13, (NB100-62126/NBP1-05763)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cystatin C

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. The cystatin superfamily encompasses proteins that contain multiple cystatin like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. Cystatin C (Cystatin 3) , a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human)

Gene Symbol

CST3

Additional Cystatin C Products

Product Documents for Cystatin C Antibody (Cyst24cc)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cystatin C Antibody (Cyst24cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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