CYTIP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1002
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence SLQRLLQHSSNGN-C corresponding to N-Terminus according to NP_004279.3.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Canine, Porcine.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CYTIP Antibody
![Western Blot: CYTIP Antibody [NB100-1002]](https://resources.bio-techne.com/images/products/CYTIP-Antibody-Western-Blot-NB100-1002-img0001.jpg "Western Blot: CYTIP Antibody [NB100-1002]")
Western Blot: CYTIP Antibody [NB100-1002]
Western Blot: CYTIP Antibody [NB100-1002] - Staining of Jurkat (A) and (1 ug/mL) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.![Immunofluorescence: CYTIP Antibody [NB100-1002]](https://resources.bio-techne.com/images/products/CYTIP-Antibody-Immunofluorescence-NB100-1002-img0002.jpg "Immunofluorescence: CYTIP Antibody [NB100-1002]")
Immunofluorescence: CYTIP Antibody [NB100-1002]
Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunofluorescence: CYTIP Antibody [NB100-1002]](https://resources.bio-techne.com/images/products/CYTIP-Antibody-Immunofluorescence-NB100-1002-img0003.jpg "Immunofluorescence: CYTIP Antibody [NB100-1002]")
Immunofluorescence: CYTIP Antibody [NB100-1002]
Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for CYTIP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3 - 1 ug/mL
Application Notes
Western blot: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in lysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CYTIP
Alternate Names
B3-1, CASPCytohesin-associated scaffolding protein, Cbp HE, CYBRCytohesin-binding protein HE, CYTHIP, cytohesin 1 interacting protein, Cytohesin binder and regulator, cytohesin-1 interacting protein, cytohesin-interacting protein, HEPleckstrin homology Sec7 and coiled-coil domains-binding protein, pleckstrin homology, Sec7 and coiled/coil domains, binding protein, pleckstrin homology, Sec7 and coiled-coil domains, binding protein, PSCDBPcytohesin binding protein HE
Entrez Gene IDs
9595 (Human)
Gene Symbol
CYTIP
UniProt
Additional CYTIP Products
Product Documents for CYTIP Antibody
Product Specific Notices for CYTIP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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