Cytochrome b5 type A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14923
Key Product Details
Species Reactivity
Validated:
Human, Canine, Feline
Predicted:
Bovine (91%), Canine (95%), Monkey (97%), Porcine (92%), Sheep (92%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytochrome b5 type A Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytochrome b5 type A. The exact sequence is proprietary.
Reactivity Notes
Rhesus Monkey (97%), Chicken (82%), Mouse (88%), Rat (88%), Xenopus laevis (80%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytochrome b5 type A Antibody
![Western Blot: Cytochrome b5 type A Antibody [NBP2-14923]](https://resources.bio-techne.com/images/products/Cytochrome-b5-type-A-Antibody-Western-Blot-NBP2-14923-img0002.jpg "Western Blot: Cytochrome b5 type A Antibody [NBP2-14923]")
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923]
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE, antibody diluted at 1:500.
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] -
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] -
Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for Cytochrome b5 type A Antibody
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cytochrome b5 type A
Alternate Names
CYB5, cytochrome b-5, cytochrome b5 (microsomal), cytochrome b5 type A (microsomal), MCB5cytochrome b5, Microsomal cytochrome b5 type A, type 1 cyt-b5
Gene Symbol
CYB5A
UniProt
Additional Cytochrome b5 type A Products
Product Documents for Cytochrome b5 type A Antibody
Product Specific Notices for Cytochrome b5 type A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.