Cytochrome b5 type A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38144
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cytochrome b5 type A (NP_683725.1).
Sequence:
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLI
Sequence:
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytochrome b5 type A Antibody
Western Blot: Cytochrome b5 type A Antibody [NBP3-38144] -
Western Blot: Cytochrome b5 type A Antibody [NBP3-38144] - Western blot analysis of various lysates using Cytochrome b5 type A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.
Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] -
Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Cytochrome b5 type A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] -
Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using Cytochrome b5 type A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Applications for Cytochrome b5 type A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytochrome b5 type A
Alternate Names
CYB5, cytochrome b-5, cytochrome b5 (microsomal), cytochrome b5 type A (microsomal), MCB5cytochrome b5, Microsomal cytochrome b5 type A, type 1 cyt-b5
Gene Symbol
CYB5A
Additional Cytochrome b5 type A Products
Product Documents for Cytochrome b5 type A Antibody
Product Specific Notices for Cytochrome b5 type A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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