Cytochrome c Antibody (7H8.2C12) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55775
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila, Rabbit
Cited:
Human, Mouse, Rat, Insect - Drosophila
Applications
Validated:
Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 7H8.2C12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Specificity
An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Marker
Mitochondria Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Cytochrome c Antibody (7H8.2C12) [Biotin]
Western Blot: Cytochrome C Antibody (7H8.2C12) [Biotin] [NB100-55775] - Western blot detection of Cytochrome C in 15 mgs of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.
Applications for Cytochrome c Antibody (7H8.2C12) [Biotin]
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
0.05-0.5 ug/ml
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytochrome c
Alternate Names
CYCS
Gene Symbol
CYCS
Additional Cytochrome c Products
Product Documents for Cytochrome c Antibody (7H8.2C12) [Biotin]
Product Specific Notices for Cytochrome c Antibody (7H8.2C12) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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