Cytochrome P450 17A1 Antibody (5A9)

Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] -

Immunohistochemistry: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Image of Cytochrome P450 17A1 Antibody (5A9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] -

Western Blot: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Positive Western Blot detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate.
All lanes: Cytochrome P450 17A1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 58 kDa
Observed band size: 58 kDa

Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] -

Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5A9) [NBP3-26637] - Staining of Hela Cells with Cytochrome P450 17A1 Antibody (5A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).