Cytochrome P450 17A1 Antibody (5D2H1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16541

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16541-100ul
NBP3-16541-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D2H1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytochrome P450 17A1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 101-220 of human Cytochrome P450 17A1 (P05093). TLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEISTLCDMLATHNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLVPW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytochrome P450 17A1 Antibody (5D2H1)

Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541]

Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541]

Western Blot: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 17A1 Antibody (5D2H1) [NBP3-16541] - Immunofluorescence analysis of HeLa cells using Cytochrome P450 17A1 Rabbit mAb (NBP3-16541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Cytochrome P450 17A1 Antibody (5D2H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 17A1

The CYP17A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The CYP17A1 protein localizes to the endoplasmic reticulum. CYP17A1 has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in the CYP17A1 gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. (provided by RefSeq, Jul 2008)

Long Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Alternate Names

CYP17, CYP17A1, CYPXVII, Cytochrome P450-C17, Cytochrome P450c17, EC 1.14.14.19, EC 1.14.14.32, S17AH

Gene Symbol

CYP17A1

Additional Cytochrome P450 17A1 Products

Product Documents for Cytochrome P450 17A1 Antibody (5D2H1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytochrome P450 17A1 Antibody (5D2H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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