Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70531
![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-OTI5B9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70531-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]")
![Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70531-img0005.jpg "Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]")
![Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9)Azide and BSA Free [NBP2-70531]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-OTI5B9-Azide-and-BSA-Free-Western-Blot-NBP2-70531-img0001.jpg "Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9)Azide and BSA Free [NBP2-70531]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5B9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free
Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9)Azide and BSA Free [NBP2-70531]
Western Blot: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cyt![Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-OTI5B9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70531-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]")
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]
Immunocytochemistry/Immunofluorescence: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2E1.![Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-OTI5B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70531-img0004.jpg "Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]")
Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531]
Immunohistochemistry: Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free [NBP2-70531] - Staining of paraffin-embedded endometrium tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.Applications for Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytochrome P450 2E1
Alternate Names
4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase
Gene Symbol
CYP2E1
Additional Cytochrome P450 2E1 Products
Product Documents for Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free
Product Specific Notices for Cytochrome P450 2E1 Antibody (OTI5B9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...