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Cytochrome P450 3A4 Antibody (3H8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37502

Novus Biologicals, part of Bio-Techne
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NBP2-37502SS
NBP2-37502

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H8

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human Cytochrome P450 3A4 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 3A4 Antibody (3H8) - BSA Free

Western Blot: Cytochrome P450 3A4 Antibody (3H8)BSA Free [NBP2-37502]

Western Blot: Cytochrome P450 3A4 Antibody (3H8)BSA Free [NBP2-37502]

Western Blot: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]

Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) - BSA Free [NBP2-37502]

Immunohistochemistry-Paraffin: Cytochrome P450 3A4 Antibody (3H8) [NBP2-37502] - Analysis of human brain tissues using CYP3A4 mouse mAb with DAB staining.

Applications for Cytochrome P450 3A4 Antibody (3H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP3A4

Long Name

Cytochrome P450 3A4

Alternate Names

Albendazole Monooxygenase, Albendazole Sulfoxidase, CP33, CP34, CYP3A3, CYPIIIA4, NF-25, P450C3, P450PCN1, Quinine 3-Monooxygenase, Taurochenodeoxycholate 6-Alpha-Hydroxylase

Gene Symbol

CYP3A4

Additional CYP3A4 Products

Product Documents for Cytochrome P450 3A4 Antibody (3H8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome P450 3A4 Antibody (3H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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