Cytochrome P450 3A4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1396

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB600-1396
NB600-1396-0.025ml
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYP3A4 Antibodies »

Product Specifications

Immunogen

Purified, His-tagged, full length human P450 3A4 fusion protein.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18189363).

Specificity

Detects Cytochrome P450 (P450) 3A4 from human and rat tissues and cells. This detects, to a lesser extent, other P450 isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytochrome P450 3A4 Antibody

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - CYP3A4 (A) and HMG-CoA (B) levels from liver samples from chronically administered animals (14 days) were quantified by ELISA and western blots. Beta-actin served as a reference signal for CYP3A4 (C) and HMG-CoA Reductase (D). Error bars signify standard error of the mean.Citation: Buchanan B, Meng Q, Poulin M-M, Zuccolo J, Azike CG, Gabriele J, et al. (2018) Comparative pharmacokinetics and safety assessment of transdermal berberine and dihydroberberine. PLoS ONE 13(3): e0194979. https://doi.org/10.1371/journal.pone.0194979
Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of rat liver extract.
Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396]

Western Blot: Cytochrome P450 3A4 Antibody [NB600-1396] - Analysis of from rat liver extract.
View 1 more images

Applications for Cytochrome P450 3A4 Antibody

Application
Recommended Usage

Western Blot

1:1000 - 1:5000
Application Notes
WB: Detects an approx. 46 kDa protein representing P450 3A4 from rat liver extracts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYP3A4

The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Long Name

Cytochrome P450 3A4

Alternate Names

Albendazole Monooxygenase, Albendazole Sulfoxidase, CP33, CP34, CYP3A3, CYPIIIA4, NF-25, P450C3, P450PCN1, Quinine 3-Monooxygenase, Taurochenodeoxycholate 6-Alpha-Hydroxylase

Entrez Gene IDs

1576 (Human)

Gene Symbol

CYP3A4

Additional CYP3A4 Products

Product Documents for Cytochrome P450 3A4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytochrome P450 3A4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.