Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # KRT1/1840

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Cytokeratin 1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 1 protein (Uniprot: P04264)

Localization

Cytoplasmic

Marker

Suprabasal Keratinocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07776).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).
Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475]

Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free [NBP3-08475] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 1 Mouse Monoclonal Antibody (KRT1/1840).

Applications for Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2-4 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 1

Belonging to the keratin family, Cytokeratin 1 plays an important role in cell binding. More specifically, this gene is expressed in the spinous and granular layers of the epidermis with keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. This gene also regulates the activity of kinases such as PKC and SRC by binding to ITGB1 and the receptor of GNB2L1. Other biological processes include complement activation, lectin pathway, epidermis development, fibrinolysis, regulation of angiogenesis and response to oxidative stress.

Alternate Names

CK1, CK-1, cytokeratin 1, Cytokeratin-1, cytokeratin-1,67 kDa cytokeratin, EHK, EHK1, epidermolytic hyperkeratosis 1, EPPK, Hair alpha protein, K1, keratin 1, keratin, type II cytoskeletal 1, Keratin-1, KRT1A, KRTA, NEPPK, Type-II keratin Kb1

Gene Symbol

KRT1

Additional Cytokeratin 1 Products

Product Documents for Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 1 Antibody (KRT1/1840) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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