Cytokeratin 10/13 Antibody (SPM262)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44710
![Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) [NBP2-44710]](https://resources.bio-techne.com/images/products/Cytokeratin-10-13-Antibody-SPM262-Immunohistochemistry-Paraffin-NBP2-44710-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) [NBP2-44710]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Feline
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM262
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Localization
Cytoplasmic
Specificity
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11558)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Cytokeratin 10/13 Antibody (SPM262)
Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) [NBP2-44710]
Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (SPM262) [NBP2-44710] - Human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (SPM262)Applications for Cytokeratin 10/13 Antibody (SPM262)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 10/13
Alternate Names
BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, Keratin-10, KPP
Gene Symbol
KRT10
UniProt
Additional Cytokeratin 10/13 Products
Product Documents for Cytokeratin 10/13 Antibody (SPM262)
Product Specific Notices for Cytokeratin 10/13 Antibody (SPM262)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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