Cytokeratin 10 Antibody (4S5Y7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16460
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4S5Y7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 485-584 of human Cytokeratin 10 (P13645). GGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 10 Antibody (4S5Y7)
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460] -
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460] - Confocal imaging of paraffin-embedded Human skin using Cytokeratin 10 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] -
Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] -Human cervix cancer tissue using Involucrin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.![Western Blot: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460]](https://resources.bio-techne.com/images/products/Cytokeratin-10-Antibody-4S5Y7-Western-Blot-NBP3-16460-img0004.jpg "Western Blot: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460]")
Western Blot: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460]
Western Blot: Cytokeratin 10 Antibody (4S5Y7) [NBP3-16460] - Analysis of extracts of various cell lines, using Cytokeratin 10 antibody (NBP3-16460) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Applications for Cytokeratin 10 Antibody (4S5Y7)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Western Blot
1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 10
Alternate Names
BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
Gene Symbol
KRT10
Additional Cytokeratin 10 Products
Product Documents for Cytokeratin 10 Antibody (4S5Y7)
Product Specific Notices for Cytokeratin 10 Antibody (4S5Y7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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