Cytokeratin 10 Antibody (SPM623)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53134
![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134]](https://resources.bio-techne.com/images/products/Cytokeratin-10-Antibody-SPM623-Immunohistochemistry-Paraffin-NBP2-53134-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine, Feline
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM623
Concentration
0.2 mg/ml
Product Summary for Cytokeratin 10 Antibody (SPM623)
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium (Uniprot: P13645)
Localization
Cytoplasmic
Marker
Suprabasal Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54332)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 10 Antibody (SPM623)
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134]
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 10 Monoclonal Antibody (SPM623).
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] -
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (SPM623) [NBP2-53134] - CK10-positive human FFPE skin tissue section. Image from verified customer review.Applications for Cytokeratin 10 Antibody (SPM623)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 10
Alternate Names
BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
Gene Symbol
KRT10
UniProt
Additional Cytokeratin 10 Products
Product Documents for Cytokeratin 10 Antibody (SPM623)
Product Specific Notices for Cytokeratin 10 Antibody (SPM623)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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