Cytokeratin 13 Antibody (5M6B10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15261
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5M6B10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytokeratin 13 Antibody (5M6B10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Cytokeratin 13 (P13646). LTGNEKITMQNLNDRLASYLEKVRALEEANADLEVKIRDWHLKQSPASPERDYSPYYKTIEELRDKILTATIENNRVILEIDNARLAADDFRLKYENELAL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 13 Antibody (5M6B10)
Western Blot: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261]
Western Blot: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261] - Western blot analysis of extracts of various cell lines, using Cytokeratin 13 (KRT13) Rabbit mAb (NBP3-15261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261]
Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261] - Immunofluorescence analysis of human cervix cancer using Cytokeratin 13 (KRT13) Rabbit mAb (NBP3-15261) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261]
Immunohistochemistry: Cytokeratin 13 Antibody (5M6B10) [NBP3-15261] - Immunofluorescence analysis of mouse bladder using Cytokeratin 13 (KRT13) Rabbit mAb (NBP3-15261) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Cytokeratin 13 Antibody (5M6B10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 13
Alternate Names
CK13, CK-13, cytokeratin 13, Cytokeratin-13, K13cytokeratin-13, keratin 13, keratin, type I cytoskeletal 13, keratin-13, MGC161462, MGC3781
Gene Symbol
KRT13
Additional Cytokeratin 13 Products
Product Documents for Cytokeratin 13 Antibody (5M6B10)
Product Specific Notices for Cytokeratin 13 Antibody (5M6B10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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