Cytokeratin 14 Antibody (KRT14/4131) [DyLight 755]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20723IR

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT14/4131

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 14 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa351-472) of human Cytokeratin 14 protein (exact sequence is proprietary)

Localization

Cytoplasm.

Specificity

Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Cytokeratin 14 Antibody (KRT14/4131) [DyLight 755]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (KRT14/4131) [DyLight 755]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 14 Antibody (KRT14/4131) [DyLight 755]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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