Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34403

Novus Biologicals, part of Bio-Techne
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NBP2-34403-0.1mg
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # SPM263

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 14 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14. (Uniprot: P02533)

Localization

Cytoplasmic

Specificity

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Marker

Squamous Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34355).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403] - Formalin-paraffin prostate (10X) stained with Cytokeratin 14 MAb (SPM263).
Flow Cytometry: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403]

Flow Cytometry: Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free [NBP2-34403] - Enrichment of Cytokeratin 14 (high cells after UTX knockdown in HBLAK cells. Cytokeratin 14 expression as detected by flow cytometry on day 4 after transfection of control siRNA or UTX-siRNA (n = 4). Statistics were performed with a two-way ANOVA with a post-hoc Tukey HSD test (## p < 0.01; ### p < 0.001). Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2072-6694/12/4/1023/htm), licensed under a CC-BY license.
View 2 more images

Applications for Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34403 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 14

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 14 Antibody (SPM263) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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