Cytokeratin 15 Antibody (9F9F0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16492
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9F9F0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 357-456 of human Cytokeratin 15 (P19012). YATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIAIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 15 Antibody (9F9F0)
![Western Blot: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]](https://resources.bio-techne.com/images/products/Cytokeratin-15-Antibody-9F9F0-Western-Blot-NBP3-16492-img0002.jpg "Western Blot: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]")
Western Blot: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]
Western Blot: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492] - Western blot analysis of extracts of Cal-27 cells, using Cytokeratin 15 (KRT15) antibody (NBP3-16492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]](https://resources.bio-techne.com/images/products/Cytokeratin-15-Antibody-9F9F0-Immunohistochemistry-NBP3-16492-img0001.jpg "Immunohistochemistry: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]")
Immunohistochemistry: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492]
Immunohistochemistry: Cytokeratin 15 Antibody (9F9F0) [NBP3-16492] - Immunofluorescence analysis of rat skin using Cytokeratin 15 (KRT15) Rabbit mAb (NBP3-16492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Cytokeratin 15 Antibody (9F9F0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 15
Alternate Names
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Gene Symbol
KRT15
Additional Cytokeratin 15 Products
Product Documents for Cytokeratin 15 Antibody (9F9F0)
Product Specific Notices for Cytokeratin 15 Antibody (9F9F0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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