Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54463
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free [NBP2-54463]](https://resources.bio-techne.com/images/products/Cytokeratin-15-Antibody-KRT15-1699-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54463-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free [NBP2-54463]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Bovine
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # KRT15/1699
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Cytokeratin 15 protein (Uniprot: P19012)
Localization
Cytoplasmic
Marker
Esophageal Squamous Cell Carcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53307).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free [NBP2-54463]
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free [NBP2-54463] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Monoclonal Antibody (KRT15/1699).Applications for Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 15
Alternate Names
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Gene Symbol
KRT15
Additional Cytokeratin 15 Products
Product Documents for Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free
Product Specific Notices for Cytokeratin 15 Antibody (KRT15/1699) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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