Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08478

Novus Biologicals, part of Bio-Techne
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NBP3-08478
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # KRT15/2957

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Cytokeratin 15 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 15 protein (Uniprot: P19012)

Localization

Cytoplasmic

Marker

Esophageal Squamous Cell Carcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07613).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free

Western Blot: Cytokeratin 15 Antibody (KRT15/2957)Azide and BSA Free [NBP3-08478]

Western Blot: Cytokeratin 15 Antibody (KRT15/2957)Azide and BSA Free [NBP3-08478]

Western Blot: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478] - Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478]

Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478] - Immunofluorescence Analysis of methanol-fixed human MCF-7 cells. Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478]

Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free [NBP3-08478] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957)
View 5 more images

Applications for Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 15

Alternate Names

CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15

Gene Symbol

KRT15

Additional Cytokeratin 15 Products

Product Documents for Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 15 Antibody (KRT15/2957) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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