Cytokeratin 17/36 Antibody (SR45-06)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67318
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR45-06
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Cytokeratin 17/36 aa 1-50 / 432. (SwissProt: Q04695 Human; SwissProt: Q9QWL7 Mouse; SwissProt: Q6IFU8 Rat)
Localization
Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 17/36 Antibody (SR45-06)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318]
Immunocytochemistry/Immunofluorescence: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318] - Staining Cytokeratin 17 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318]
Immunohistochemistry-Paraffin: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 17/36 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5Flow Cytometry: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318]
Flow Cytometry: Cytokeratin 17/36 Antibody (SR45-06) [NBP2-67318] - Analysis of Hela cells with Cytokeratin 17 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Applications for Cytokeratin 17/36 Antibody (SR45-06)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:200
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 17/36
Alternate Names
39.1, CK-17, Cytokeratin-17, K17, keratin 17, keratin, type I cytoskeletal 17, Keratin-17, PC, PC2, PCHC1
Gene Symbol
KRT17
Additional Cytokeratin 17/36 Products
Product Documents for Cytokeratin 17/36 Antibody (SR45-06)
Product Specific Notices for Cytokeratin 17/36 Antibody (SR45-06)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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