Cytokeratin 17 Antibody (KRT17/8346R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20320

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT17/8346R

Concentration

0.2 mg/ml

View all Cytokeratin 17 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Cytokeratin 17 protein (exact sequence is proprietary)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: T24 cells. Human skin, bladder or cervix.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Cytokeratin 17 Antibody (KRT17/8346R)

Cytokeratin 17 Antibody (KRT17/8346R)

Cytokeratin 17 Antibody (KRT17/8346R)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Cytokeratin 17 antibody (KRT17/8346R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Cytokeratin 17 Antibody (KRT17/8346R)

Cytokeratin 17 Antibody (KRT17/8346R)

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 17 antibody (KRT17/8346R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications for Cytokeratin 17 Antibody (KRT17/8346R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody (KRT17/8346R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 17 Antibody (KRT17/8346R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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