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Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34425

Novus Biologicals, part of Bio-Techne
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NBP2-34425-0.1mg

Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine, Goat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM560

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The cytoskeletal fraction of rat colon epithelium

Localization

Cytoplasmic

Specificity

Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as 'epithelial stem cell' marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK56, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

Marker

Basal Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34378).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

Western Blot: Cytokeratin 17 Antibody (SPM560)Azide and BSA Free [NBP2-34425]

Western Blot: Cytokeratin 17 Antibody (SPM560)Azide and BSA Free [NBP2-34425]

Western Blot: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425] - Western blot of HeLa lysate using CK17 Ab (SPM560).
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425] - Formalin-paraffin skin stained with CK17 MAb (SPM560).
Flow Cytometry: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425]

Flow Cytometry: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425]

Flow Cytometry: Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free [NBP2-34425] - Flow Cytometric Analysis of PFA-fixed HeLa cells labeling CK17 with Cytokeratin 17 Antibody (SPM560) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

Applications for Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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