Cytokeratin 17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38536

Novus Biologicals, part of Bio-Techne
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NBP2-38536
NBP2-38536-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 17 Antibodies »

Product Summary for Cytokeratin 17 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SSRTSCRLSGGLGAGSCRLGSAGGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 17 Antibody

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536]

Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Analysis in human urinary bladder and liver tissues using NBP2-38536 antibody. Corresponding Cytokeratin 17 RNA-seq data are presented for the same tissues.
Western Blot: Cytokeratin 17 Antibody [NBP2-38536]

Western Blot: Cytokeratin 17 Antibody [NBP2-38536]

Western Blot: Cytokeratin 17 Antibody [NBP2-38536] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - MYP3 rat urothelial cell line with CK17 (green) and DAPI (blue). Primary antibody at 1:200. Image from verified customer review.
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Applications for Cytokeratin 17 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-38536 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

UniProt

Q04695 (Human)

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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