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Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48075

Novus Biologicals, part of Bio-Techne
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NBP2-48075

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Equine, Feline, Ferret, Goat, Hamster, Monkey, Sheep, Xenopus (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C-04 (same as Ks18.04)

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Reactivity Notes

Marmoset Monkey. Does not react with Chicken or Xenopus.

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (C-04).

Applications for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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