Cytokeratin 18 Antibody (DA7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29409

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DA7

Concentration

0.2 mg/ml

View all Cytokeratin 18 Antibodies »

Product Summary for Cytokeratin 18 Antibody (DA7)

Immunogen

Human breast cancer PMC 42 cells

Reactivity Notes

Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33176)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 18 Antibody (DA7)

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.
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Applications for Cytokeratin 18 Antibody (DA7)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 18

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Cytokeratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given cytokeratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin. The DA7 antibody clone reacts with Keratin 18 (CK18), a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Cytokeratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

UniProt

P05783 (Human)

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (DA7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody (DA7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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