Cytokeratin 18 Antibody (DC10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29461

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Bovine (Negative), Canine (Negative), Ferret (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DC10

Concentration

0.2 mg/ml

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

PMC-42 human breast carcinoma cells

Reactivity Notes

Does not react with Mouse, Rat, Ferret, Hamster, Sheep, Bovine, Canine or Porcine.

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33082)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 18 Antibody (DC10)

Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DC10).
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.
Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461]

Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Detection of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
View 3 more images

Applications for Cytokeratin 18 Antibody (DC10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 18

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Keratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given keratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin.

The DC10 antibody clone reacts with Keratin 18, a 45 kDa molecular weight polypeptide that is expressed in combination with Keratin 8. Keratin 18 and 8 are the most commonly expressed intermediate filaments in single layer or simple epithelial tissues. The antibody recognizes a wide variety of simple epithelia, including glandular epithelium, but not stratified squamous epithelia (Lauerova et al, 1988).

Keratin 18 is often persistently expressed in tumor cells derived from simple epithelia, and the DC10 antibody has been useful in studies for defining tissue origin (Lane et al, 1990, Kovarik et al, 1988). For example, a cheek mass was found to be negative for the keratin 18 antibody, helping to rule out an epithelial cancer (Terada, 2011). The antibody has also been used define keratin expression patterns and develop markers for tumor subtyping. For example, antibody staining results suggested that differential expression of keratin 18 in jaw tumors may useful in distinguishing ameloblastic carcinomas (keratin 18 positive) from ameloblastomas (keratin 18 negative).

The antibody is widely published; researchers are encouraged to perform a key word search on scholar.google.com for additional information about how it has been used.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

UniProt

P05783 (Human)

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (DC10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody (DC10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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