Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71086

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]

Western Blot: Cytokeratin 18 Antibody (OTI1E1)Azide and BSA Free [NBP2-71086]

Western Blot: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT18.
Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086]

Immunohistochemistry: Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free [NBP2-71086] - Staining of paraffin-embedded Human liver tissue within the normal limits. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, (1:6000).
View 15 more images

Applications for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody (OTI1E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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