Cytokeratin 18 Antibody (SPM265)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34356
![Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]](https://resources.bio-techne.com/images/products/Cytokeratin-18-Antibody-SPM265-Western-Blot-NBP2-34356-img0002.jpg "Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]")
![Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]](https://resources.bio-techne.com/images/products/Cytokeratin-18-Antibody-SPM265-Western-Blot-NBP2-34356-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM265
Concentration
0.2 mg/ml
Product Summary for Cytokeratin 18 Antibody (SPM265)
Immunogen
PMC-42 human breast carcinoma cells
Reactivity Notes
Does not react with Mouse, Rat, Sheep, Hamster, Bovine, Canine or Porcine.
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34404)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 18 Antibody (SPM265)
Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]
Western Blot: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (SPM265).![Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]](https://resources.bio-techne.com/images/products/Cytokeratin-18-Antibody-SPM265-Immunocytochemistry-Immunofluorescence-NBP2-34356-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]
Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Confocal Immunofluorescence of HeLa cells Cytokeratin 18 Antibody (SPM265). labeled with CF488 (Green); Red Dot is used to label the nuclei.![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]](https://resources.bio-techne.com/images/products/Cytokeratin-18-Antibody-SPM265-Immunohistochemistry-Paraffin-NBP2-34356-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]")
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) [NBP2-34356]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SPM265) [NBP2-34356] - Formalin-paraffin skin sweat gland stained with Cytokeratin 18 MAb (SPM265). Note cytoplasmic staining of tumor cells.Applications for Cytokeratin 18 Antibody (SPM265)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (SPM265)
Product Specific Notices for Cytokeratin 18 Antibody (SPM265)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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