Cytokeratin 18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90353

Novus Biologicals, part of Bio-Techne
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NBP1-90353
NBP1-90353-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEINLDSMRNLKASLENSLREVVARYALQMEQLNGILLHLESELAQTWAEGQCQAQEYQALLNIKVKLEAAIATYRRLLEDG

Marker

Epithelial Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 18 Antibody

Western Blot: Cytokeratin 18 Antibody [NBP1-90353]

Western Blot: Cytokeratin 18 Antibody [NBP1-90353]

Western Blot: Cytokeratin 18 Antibody [NBP1-90353] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT18 antibody. Corresponding KRT18 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353]

Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP1-90353] - Staining of human small intestine shows moderate cytoplasmic and membrane positivity in glandular cells.
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Applications for Cytokeratin 18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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