Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E8

Format

BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 19 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Cytokeratin 19 expressed in E. coli. [UniProt# P08727]

Marker

Epithelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 19 Antibody (4E8) - BSA Free

Western Blot: Cytokeratin 19 Antibody (4E8)BSA Free [NBP2-22116]

Western Blot: Cytokeratin 19 Antibody (4E8)BSA Free [NBP2-22116]

Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (4E8) - BSA Free [NBP2-22116]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (4E8) - BSA Free [NBP2-22116]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunofluorescence analysis of HepG2 cells using Cytokeratin 19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) - BSA Free [NBP2-22116]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) - BSA Free [NBP2-22116]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.
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Applications for Cytokeratin 19 Antibody (4E8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:25-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:500-1:2000
Application Notes
This Cytokeratin 19 (4E8) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 19

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

Alternate Names

CK19, EndoC, K19, Krt19

Entrez Gene IDs

3880 (Human)

Gene Symbol

KRT19

UniProt

P08727

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (4E8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 19 Antibody (4E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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