Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33134MFV500
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Ferret (Negative), Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Monoclonal Mouse IgG2a Lambda Clone # A53-B/A2.26
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human breast cancer MCF-7 cells
Epitope
aa 312-335
Reactivity Notes
Does not react with Rat or Ferret.
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Marker
Pancreatic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Lambda
Scientific Data Images for Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE]
Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE] -
Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications for Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cytokeratin 19
Alternate Names
CK19, EndoC, K19, Krt19
Gene Symbol
KRT19
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE]
Product Specific Notices for Cytokeratin 19 Antibody (A53-B/A2.26) [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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