Cytokeratin 19 Antibody (KRT19/1959R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07693
Recombinant Monoclonal Antibody
![Western Blot: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]](https://resources.bio-techne.com/images/products/Cytokeratin-19-Antibody-KRT19-1959R-Western-Blot-NBP3-07693-img0002.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # KRT19/1959R
Concentration
0.2 mg/ml
Product Summary for Cytokeratin 19 Antibody (KRT19/1959R)
Immunogen
Recombinant human full-length Cytokeratin 19 protein (Uniprot: P08727)
Localization
Cytoplasmic
Specificity
This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Marker
Pancreatic Stem Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08635)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 19 Antibody (KRT19/1959R)
Western Blot: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]
Western Blot: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693] - Western Blot Analysis of Hep-G2 cell lysate using Cytokeratin 19 Rabbit Recombinant Monoclonal Antibody (KRT19/1959R).![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]](https://resources.bio-techne.com/images/products/Cytokeratin-19-Antibody-KRT19-1959R-Immunohistochemistry-Paraffin-NBP3-07693-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]")
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693]
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/1959R) [NBP3-07693] - Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 19 Rabbit Recombinant Monoclonal Antibody (KRT19/1959R).Applications for Cytokeratin 19 Antibody (KRT19/1959R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 19
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody (KRT19/1959R)
Product Specific Notices for Cytokeratin 19 Antibody (KRT19/1959R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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