Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34405

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda/IgG1 Kappa Clone # SPM266

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 19 Antibodies »

Product Specifications

Immunogen

Human breast cancer MCF-7 cells

Epitope

aa 312-335

Localization

Cytoplasmic

Specificity

This monoclonal antibody reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Marker

Pancreatic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda/IgG1 Kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34357).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free

Western Blot: Cytokeratin 19 Antibody (SPM266)Azide and BSA Free [NBP2-34405]

Western Blot: Cytokeratin 19 Antibody (SPM266)Azide and BSA Free [NBP2-34405]

Western Blot: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405] - Western Blot Analysis of human Hep-G2 cell lysate using Cytokeratin 19 Antibody (SPM266).
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with cytokeratin 19 Monoclonal Antibody (SPM266).
Flow Cytometry: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405]

Flow Cytometry: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405]

Flow Cytometry: Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free [NBP2-34405] - Flow Cytometric Analysis of Methanol-fixed MCF-7 cells. Cytokeratin 19 Antibody (SPM266) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 19

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Alternate Names

CK19, EndoC, K19, Krt19

Gene Symbol

KRT19

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 19 Antibody (SPM266) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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