Cytokeratin 19 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78278

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.16 mg/ml

View all Cytokeratin 19 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human Cytokeratin 19 protein (between residues 1-50) [UniProt P08727]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27070090).

Localization

Cytoplasmic.

Marker

Epithelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 19 Antibody - BSA Free

Western Blot: Cytokeratin 19 AntibodyBSA Free [NBP1-78278]

Western Blot: Cytokeratin 19 AntibodyBSA Free [NBP1-78278]

Western Blot: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in MCF7 lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NBP1-78278]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NBP1-78278]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NBP1-78278] - Antibody at 1:25 in MCF7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Cytokeratin 19 Antibody - BSA Free [NBP1-78278]

Immunohistochemistry: Cytokeratin 19 Antibody - BSA Free [NBP1-78278]

Immunohistochemistry: Cytokeratin 19 Antibody [NBP1-78278] - Analysis of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.
View 2 more images

Applications for Cytokeratin 19 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:75

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Simple Western

1:3000

Western Blot

2 ug/mL
Application Notes
In Western blot a band is seen at ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-78278 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.16 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 19

Cytokeratin 19 (KRT19) is an intermediate filament (IF) protein that is part of the Keratin family of proteins. This protein is also known by the names of Keratin 19 or Keratin type I Cytoskeletal 19. The Keratin family consists of Keratin 1-20, which are divided into two subtypes: Type 1 contains the acidic Cytokeratin proteins consisting of Cytokeratins 9-20, including KRT19; Type 2 Keratins include the basic to neutral protein and contains Cytokeratin 1-8. Type 1 and Type 2 Keratins often form oligomers, interacting to create heterotypic chains. However, KRT19 is unique in that while being the smallest of the Type 1 Keratin proteins, it does not interact with any Type 2 Keratins. IF proteins such as KRT19 are integral for defining and maintaining cellular structure and morphology, and are important for cellular integrity by anchoring to membrane complexes via proteins such as Epiplakin, Trichoplein, Periplakin, Plakophilins, Plectin, BPAG1, Desmoplakin, and Envoplakin. KRT19 is also involved in the organization of the myofibers of striated muscle. KRT19 and other Cytokeratin proteins are mainly found in epithelial cells, with KRT19 being expressed exclusively in defined zones of basal keratinocytes. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.

Alternate Names

CK19, EndoC, K19, Krt19

Entrez Gene IDs

3880 (Human)

Gene Symbol

KRT19

UniProt

P08727

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 19 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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