Cytokeratin 20 Antibody (10D8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42616

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 10D8

Format

BSA Free

Concentration

1 mg/ml

View all Cytokeratin 20 Antibodies »

Product Specifications

Immunogen

Recombinant human Cytokeratin 20 (1-424aa) purified from E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Cytokeratin 20 Antibody (10D8) - BSA Free

Western Blot: Cytokeratin 20 Antibody (10D8)BSA Free [NBP2-42616]

Western Blot: Cytokeratin 20 Antibody (10D8)BSA Free [NBP2-42616]

Western Blot: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human KRT20 Lane 2.: HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10D8) - BSA Free [NBP2-42616]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (10D8) - BSA Free [NBP2-42616]

Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - Analysis of Cytokeratin 20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Cytokeratin 20 Antibody (10D8) - BSA Free [NBP2-42616]

Flow Cytometry: Cytokeratin 20 Antibody (10D8) - BSA Free [NBP2-42616]

Flow Cytometry: Cytokeratin 20 Antibody (10D8) [NBP2-42616] - An intracellular stain was performed on HeLa cells with Cytokeratin 20 Antibody (10D8) NBP2-42616AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 format of this antibody.

Applications for Cytokeratin 20 Antibody (10D8) - BSA Free

Application
Recommended Usage

Western Blot

1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

UniProt

NP_061883 (Human)

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (10D8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 20 Antibody (10D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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