Cytokeratin 20 Antibody (2G3-1C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00054474-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G3-1C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 20 Antibodies »

Product Specifications

Immunogen

KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

Reactivity Notes

Other species not tested.

Specificity

KRT20 - keratin 20

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cytokeratin 20 Antibody (2G3-1C8)

Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - KRT20 monoclonal antibody (M01), clone 2G3-1C8 Analysis of KRT20 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on U-2 OS cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.
View 3 more images

Applications for Cytokeratin 20 Antibody (2G3-1C8)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Entrez Gene IDs

54474 (Human)

Gene Symbol

KRT20

OMIM

608218 (Human)

UniProt

AAH31559 (Human)

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (2G3-1C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 20 Antibody (2G3-1C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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